Efeito do laser de baixa potência associado ao uso de feltro ativado no processo de reparo ósseo em tíbia de ratos

Abstract

The bone defects are caused by several factors and the repair process can be slow, where inflammatory and proliferative phases of remodeling of this tissue are extremely important for the quality of the repair. There are several therapies where the main is the surgery and in this case, the use of bone substitutes can be indicated. However the repair process may require assistance. Thus, the use of activated charcoal as bone substitute when the implementation of low-power laser to aid bone repair can be an alternative to these problems. The aim of this study was to verify the use of activated carbon felt as bone substitute and the interaction with the laser in the process of bone repair in rat tibias, assessing biochemical, histological and biomechanical changes. Material and methods: male wistar rats, 35 were used between 150 g to 200 g, with 3 months of age. The animals were anesthetized with ketamine hydrochloride and Association (90mg/Kg and 10mg/Kg, IP). Once anesthetized, the animals were put on surgical table, the skin was dissected and were made monocortical bone defects in the central region of the right tibia of mice. Immediately after surgery the rats were randomized and divided into the following groups: control (CTL), untreated Injury (NT), Lesion treated with activated carbon felt (AF), Lesion treated with lasertherapy (L6J) and Lesion treated with association of activated carbon felt and laser 830nm, 6J-100mW (AF+L). After 28 days the animals were euthanized with overdose of the same anesthetic, blood was collected and the tibia was taken for biochemical, histological and biomechanical analysis. Committee of ethics: AN00462014-UNINOVE. Results: the NT group reduction in bending forces and histological changes related to disorganization of the tissue. The group showed no L improve mechanical properties, whereas AF increase the strain in compare to CTL. AF+L group presented better flexion forces, related with improvement of histological aspects, however increased the alkaline phosphatase levels. Conclusion: the use of activated carbon felt seems to improve the bone repair induced in this study. The laser association with activated carbon felt showed improvement of biomechanical properties and still the histological results have a better aspect.