Papel da via de sinalização SOCS-JAK-STAT nos efeitos anti-inflamatórios do treinamento físico aeróbio em modelo experimental de asma

Abstract

Asthma is a chronic airway inflammatory disease that results in impairment of quality of life. Studies have demonstrated that aerobic training (AT) performed properly results in anti-inflammatory effects to the airways. However, the possible extra and intracellular mediators underlying the effects of AT on asthma never were investigated, except to NF-kB. Thus, the present project aims to evaluate the effects of AT on SOCS, JAK and STAT proteins signaling on the lungs as possible modulators of the anti-inflammatory effects of aerobic training in an experimental model of asthma using HDM (House dust mite). For this purpose, the animals received intra-tracheal administration of HDM (100ug) on days 0, 7, 14, 21, 28, 35 e 42 and were submitted to AT (for evaluation of therapeutic effect) begining on day 17 during 5 weeks. Twenty-four hours after the last session of training and allergen challenge, the animals were assessed to the inflammatory profile in the bronchoalveolar lavage and in the lung tissue, to the levels of pro-inflammatory cytokines, the levels of anti-inflammatory cytokines and also for the expression of SOCS1, SOCS2, SOCS3, JAK2, STAT3, STAT5 e STAT6 in the lungs, as well as for the airway remodeling, and bronchial hyperresponsiveness. Were found increase of the LAB cells and serum, as well as the pro-inflammatory cytokines, collagen and elastic fibers in HDM group compared with the other. The TFA was capable of increasing IL-10 levels in the LAB, being able to reverse many of the structural and inflammatory aspects of asthma.