Efeitos do tratamento com vitamina D e/ou fotobiomodulação sobre o curso da fibrose pulmonar

Abstract

A high incidence of intentional or accidental ingestion of paraquat (PQ) is related to the development of irreversible pulmonary fibrosis (PF). PF is a chronic and progressive disease characterized by injury to the lung parenchyma, inflammatory infiltrate and interstitial fibrosis. It is triggered by excessive and disordered deposition of collagen and other extracellular matrix components, resulting in severe changes in alveolar wall architecture. Due to the absence of an effective treatment, the objective of the study was to investigate the effect of treatment with vitamin D and/or photobiomodulation on the course of PF. C57BL6 mice were submitted to the induction of PF by the administration of Paraquat (10mg / kg, ip) and after 7 days of induction, the mice were treated with Vitamin D (5μg / kg, ip) and /or Photobiomodulation with LED (1x / day, 5 consecutive days, 150s). Treatment with vitamin D alone reduced the number of leukocytes in BAL as well as levels of IL-6, IL-17, TGF-beta, MMP-9, collagen deposition in lung tissue and tracheal reactivity. Furthermore, treatment with vitamin D increased levels of resolvin D1 in the BAL. Photobiomodulation reduced the number of cells in the BAL and increased the levels of resolvin D1. We also observed that the association between photobiomodulation and vitamin D did not cause an additive effect that justifies its use concomitantly. Thus, we conclude that in PQ-induced pulmonary fibrosis the treatment with vitamin D alone was more efficient than photobiomodulation and may emerge as a promising treatment tool for pulmonary fibrosis