Efeito do anabolizante decanoato de nandrolona na proliferação, adesão e diferenciação de osteoblastos

Abstract

Osteoporosis is an important public health problem worldwide. The study of factors that may influence bone regeneration is of fundamental importance in the prevention of osteoporosis and clinical rehabilitation of elderly patients. Because anabolic steroids have been indicated for the treatment of such cases, the objective of this study was to evaluate the in vitro effects of the anabolic steroid nandrolone decanoate at concentrations of 5, 10, 25, and 50 M on the proliferation, adhesion and differentiation of osteoblasts (osteo-1 cell line). For the analysis of cell proliferation, growth curves were obtained after 24, 48 and 72 hours of culture using the MTT method. This same method was used to evaluate the effects of the anabolic steroid on cell adhesion 20, 40 and 60 minutes after plating. However, cell differentiation was evaluated by measuring alkaline phosphatase enzyme concentration in lysed cells after 72 hours of culture, using a specific commercial kit. Untreated cultures were used as controls. For each experimental condition, 3 experiments were performed in quadruplicate. Experimental data were statistically analyzed using analysis of variance (ANOVA) followed by Tukey s post-hoc multiple comparison test (p  0.05). The results showed that there was a significant decrease in the proliferation of osteoblasts treated with 50 M nandrolone decanoate after 72 hours of culture; however, cell differentiation increased significantly in this group compared with the control group. Also, a significant increase in cell adhesion was found in the groups treated with 25 M nandrolone decanoate after 40 and 60 minutes of incubation. It was observed that the treatment with anabolic steroid at a concentration of 50 M increased osteoblast differentiation, but not cell differentiation. Therefore, further studies on the effects of nandrolone decanoate on bone tissue using in vivo and in vitro models are necessary to determine its appropriate use and dosage in the prevention and treatment of osteoporosis.