Efeitos a curto, médio e longo prazo da combinação de laser super-pulsado e diodo emissor de luz

Abstract

It's known that photobiomodulation is the capacity to stimulate or inhibit the chemical or physiological functions of a cell, by the emission of light on the tissue, the magnitude of stimulation and/or inhibition is influenced by the physical characteristics of the light. Our hypothesis is that the phototherapy in rats is capable to promote antiaging as well as adverse effects, since scientific evidences show that it can reduce the oxidative stress, reactive oxygen species, as well as raising the levels of antioxidants. Although there are several studies about phototherapy, currently there is no research records about its antiaging and/or adverse effects, since no study, until the present moment, focused on the investigation of adverse effects related to the use of therapy for a long period of time. Therefore, the objective of this experiment is to verify the antiaging and adverse effects of phototherapy with combination of super-pulsed laser and LED, using different doses and treatment periods, when applied punctually in the anterior tibial muscle of rats. The experiment was be conducted with six-weeks-old male Wistar rats weighing 250g, divided in 5 groups, being one placebo and the others irradiated with 1, 3, 10 and 30J, each experimental group consisting of eight animals. All groups was be treated for 15 ,30 or 60 consecutive weeks, three times a week. No histological changes were found in the studied tissues; in biochemical analysis, we did not observe hepatic alterations, however, it is believed that there is an antiaging effect on renal (creatinine) and muscle (VEGF) marker.