Avaliação dos efeitos do laser diodo 808 nm em alta potência na remineralização de lesão de cárie dental induzida em dentina bovina

Abstract

This work aimed to evaluate the effects of high power diode laser, in the remineralization of the dentin caries lesion. Bovine dentine blocks were used to induce caries that were incubated in BHI broth with Streptococcus mutans ATCC 25175. The experimental groups were composed of 2 variation factors: treatment (fluorotherapy and irradiation with diode laser) and evaluation time post treatment (initial, 7, 14 and 28 days). A diode laser (Therapy, DMC, São Carlos, Brazil) was used, adjusted to continuous irradiation (CW) and 1 W of output power, blurred in an area of 0.5 cm2. After the cariogenic challenge, the specimens were evaluated in their healthy portions and affected by caries lesions and the results were compared. The specimens were divided into 4 groups (n = 12) and treated with acidulated fluorine phosphate and laser diode with a wavelength of 808 nm. The experimental groups were divided: LF: caries lesion, treated with diode and fluoride laser; F: caries and fluoride injury; FL: caries lesion, treated with fluoride and diode laser; L: caries lesion and diode laser treated. The analysis of the mineral content of the dentin was performed using the OCT images at the times: initial, 7, 14 and 28 days. The data were for analysis of variance of two factors (ANOVA Two way) and Bonferroni test for comparison between the means, which were considered different when p<0.05. Statistical differences were observed between groups and times, the maximum remineralization potential was achieved by all groups at 14 days and remained stable until the 28th. The order of treatments influenced the speed of remineralization and the groups followed by laser or fluoride irradiation irradiation have already achieved remineralization 7 days after treatment. In our experimental model, the maximum remineralization potential was achieved by all groups at 14 days. In addition, the order of treatments influenced the rate of remineralization, with fluoride followed by laser the best remineralization behavior.