Avaliação do espectro de absorção e da viabilidade de linhagens celulares cultivadas sob estresse nutricional e submetidas à fotobiomodulação

Abstract

Absorbance is the fraction of light energy that is absorbed by a specific thickness of a material. It means the ability of a material os absorbing light. In a cell cultura where photobiomodulation is used to biostimulate or bioinhibit cell reactions, the knowlogde spctrum of cell lines (HACAT, DOK, fibroblasts, CA1, LUC4 and SCC9) cultivated under a nutricional stress before and after a PBM and analyze the cell viability thorugh the assay MTT. The cell lines was cultured in médium DMEM-F12 with 10% FBS and suplementes, to evaluate the effect od nutritional stress on the light absorption spectra, cells were cultured for 24 h under the following conditions: DMEM-F12 +10% FBS, DMEM-F12 + 5% FBS and DMEM-F12. After this period, 105 cells from each experimental condition were collectes and ressuspended in 1 ml of phosphate buffer solution (PBS) to measure the absorbance in the spectrophotometer. The cells lines that showed statical difference in absorption spectrum were subjected to FBM with LED in the parameters: 6J, 660nm, 240 s. Two treatments were performed: in the first, the cells were cultivated in 48 hours under nutritional stress and through a single irradiation; the other, the same cell lines stay 96 hours under nutritional stress and were treated for three consecutive days with the LED. There is no difference between CA1, LUC4 and fibroblasts when cultivated for 24 hours in nutritional stress. The cell lines HACAT, DOK and SCC9 showed higher absorption when cultivated with higher FBS concentration. There is no statistically diferences between HACAT, DOK and SCC9 cell lines treated with one or three irradiations when cultivated for 48 and 96 houres under nutritional stress. Nutritional stress of the evaluated cell lines showed no effect on absorbance before or after PBM, nor on cell viability.