Fotobiomodulação de macrófagos e de mediadores envolvidos no processo inflamatório do tecido muscular

Abstract

Inflammatory cells invade muscle tissue after the occurrence of injury and orchestrate the tissue repair process. Among these cells, macrophages play a major role. Low-level laser (LLLT) has been widely used in clinical practice to accelerate the repair of muscle tissue, but little is known regarding its effect on macrophages and the implications of this modulation with regard to muscle cells. The aim of the study proposed is to analyze the effects of LLLT on macrophage mitochondrial activity (MA) and the production of cytokines and enzymes by activated macrophages .Macrophages of the lineage J774 was used in this study, which was activated to simulate an inflammatory situation and received two different laser irradiation parameters (780 nm, 70 mW, 3 J/cm2 and 660 nm, 15 mW, 7.5 J/cm2). The methodology consisted of macrophage cellular mitochondrial activity assays and analyses of the production of TNF-α, IL-6, IL-10 and COX-2. Non-irradiated cells served as controls. Three independent experiments were performed under each condition and the results were statistically analyzed. The LLLT (at 660 and 780 nm) was able to modulate positively the MA of activated macrophages after 3 days of incubation. The 780 nm laser decreased the production of IL-6 in activated cells after 1 day and after 5 days in non activated cells. The other proteins were not detected in supernatants. LLLT was able to modulate both mitochondrial activity as the production of IL-6 in activated macrophages. Thereby to understand the effect of LBI on muscle injury, it is important to further evaluate the role of this resource on this cell type.