Efeito da fototerapia em mioblastos c2c12 cultivados na presença de lipopolissacarídeo bacteriano

Abstract

The low level-laser therapy (LLLT) is widely used in clinical practice in order to accelerate the repair process of skeletal muscle tissues. After muscle injury resulting from infections, the process of muscle repair begins, which requires the activation of satellite cells (SC). Lipopolysaccharides (LPS) are commonly used to mimic infectious processes, and its administration into muscle cells induces changes in muscle physiology. The objective of the presente study was to evaluate the effect of LLLT on mitochondrial activity, synthesis of nitric oxide (NO), and the production of the inflammatory cytokine IL - 6. Lineage of C2C12 myoblast in differentiation were used in this study, treated with LPS (infection simulation), and laser radiation with two different parameters (780 nm; beam spot of 0.04 cm2, output power of 10 and 40 mW, energy density of 5J/cm2 and 20J/cm2 respectively and 20-second exposure time) Non-irradiated cells were used as controls, and tests performed independently. The results showed that there was an increase of mitochondrial activity in both LPS groups compared to controls, and the treatment with LLLT induced a reduction of mitochondrial activity, and modulated the synthesis of NO in myoblast but didi not alter IL-6 expression. Therefore, we conclude that LLLT is capable of modulating mitochondrial activity and the production of NO in LPS activated mioblastos, and thus may be considered as a possible tool in the treatment of muscle injury during infections.