Fototerapia pré-operatória em fibroblastos pulpares expostos aos adesivos dentinários: avaliação do efeito na modulação inflamatória

Abstract

The objective of this study was to evaluate the cytotoxicity of adhesive systems, SingleBond Universal (SB) and Optibond S (B) in pulp fibroblasts and the effect of inflammatory biomodulation low intensity laser (LBI) diode. The parameters were: power 30 mW, time 60 seconds / well, beam area 0.04 cm² and wavelengths 660 and 780 nanometers. The groups were divided into the Control Group; Group Control laser 660 nm; Group 780nm laser control; Control Group Single Bond (SB) without irradiation; Control Group Optibond (OP) without irradiation; Group SB + 660 nm; Group OP + 660 nm; Group SB + 780 nm and 780 nm + OP Group. Cytotoxicity was assessed at 6 and 24 hours after stimulation with adhesives. Cytotoxic evaluation MTT test was used for analysis and cytokine levels (TNF-α, IL-1β, IL-6, IL-8, IL-10) ELISA. Data were analyzed using SigmaStat 3.1 software (California, USA). The distribution normality was assessed using the Kolmogorov-Smirnov test and the data analyzed statistically by applying one-way ANOVA and Newman-Keuls post-test and one-way ANOVA onRanks followed by Dunn's test (P0, 05). The graphics were prepared using the software GraphPadPrism 3.1 (California, USA). Our results suggest that the materials studied have performance compatible for employment, however, caution is needed. A better understanding of the LBI role in the modulation of inflammatory mediators can be a basis for future therapeutic interventions. A more comprehensive analysis should be made with other parameters for determining a pulp cytoprotection protocol against the dentin bonding.