Efeito do laser de baixa intensidade (LBI) sobre a atividade funcional de fibroblastos de polpa de dente expostos ao adesivo odontológico experimental

Abstract

The adhesive to be ideal must present biocompatibility, join dentin and enamel, resist fracture, has good mechanical properties, be resistant to degradation in the oral environment and easy to apply. The evaluation of biocompatibility is of utmost importance since the stickers remain long in close contact with vitalized dentin. To this end, some researchers adopt cytotoxicity testing in animal models or cell cultures. Thus, the aim of this study was to evaluate the cytotoxic effect of adhesive systems used in dental practice in human dental pulp and the influence of biomodulation in the inflammatory response with low intensity lasers (LLLT). Irradiation was performed with a diode laser with 30 mW of power parameters, time 60 seconds / well, beam area of 0.04 J / cm² and wavelengths of 660 and 808 nanometers. Fibroblasts pulp FP5 commercial strain were cultured in bottles until cells reach the optimal amount and then were distributed in cell culture dishes. The groups were divided into: Basal Group; Laser Group 660nm; Laser Group 808nm; Group Round 1; Group Round 2; AD1 Group + laser 660nm; AD1 Group + laser 808nm; AD2 Group + laser 660nm; AD2 Group + 808nm laser. Cytotoxicity was assessed at 12 and 24 hours after challenge with the adhesive. The assessment of cell death was performed by flow cytometry analysis and cytokine levels (TNF-α, IL-1β, IL-6, IL-10) by ELISA. We observed a significant reduction when using the laser (660 to 808 nm) compared to group 1 and Round 808nm laser with respect to the adhesive 2. Further, we found reduced cell death either by necrosis and by apoptosis using adhesive 2, the best response using the LBI was 660nm with both adesivo1 as in 2. so, we suggest that the adhesive with increased permeability (Adesivo2) causes less cell death and activation of fibroblasts, thus possibly helping in the resolution of inflammation and tissue repair.