Efeito da fotobiomodulação associado com diclofenaco na redução do processo inflamatório e na alodinia em modelo experimental de tendinite induzida por colagenase

Abstract

The pain caused by tendinitis is frequent and difficult to treat even though there are a variety of therapies and treatments to address the pain and reduce the inflammatory process. The most commonly used therapy today is the use of anti-inflammatories that present analgesic results, but with many undesirable effects due to prolonged and indiscriminate use. Photobiomodulation therapy is already used, although on a smaller scale, but with important effects on the modulation of the acute and chronic inflammatory process with no adverse effects. The present study aimed to evaluate and compare the action of diclofenac associated with photobiomodulation therapy on the IL6 and IL10 gene expression, allodynia and the reduction of inflammatory infiltrate. Twenty-five male Wistar rats were distributed into 5 groups: Control with healthy tendon (CTL), or with collagenase- induced tendinitis (1mg/ml transcutaneous injection on calcaneous tendon). The groups with tendinitis were classified as Untreated Tendonitis (NT), Tendinitis and treated with Sodiun Diclofenac 1mg/kg intramuscular (DIC), Tendinitis and treated with Low Level Laser Irradiation (L3J) and Tendinitis and treated with a combination of Sodiun Diclofenac and Laser (D + L). After 12h of tendinitis induction, mechanical allodynia (Radal Selito) was evaluated and then the animals were euthanized and the tendon samples were removed for molecular and histological analysis. The NT group showed an increase in IL6 and IL10 gene expression, but with increase in allodynia and inflammatory infiltration with tendinous fiber disorganization. The DIC group showed an increase in the expression of IL6 and 10, and reduction of allodynia, with little improvement of the inflammatory infiltrate. Both groups L3J and D + L presented reduction in the expression of IL6 and increase of IL10, with reduction of allodynia and improvement in histological aspects. The D + L group presented significant reduction in the inflammatory process compared to the other groups treated. Conclusion: The use of photobiomodulation therapy (830nm; 3J; 100mW) showed an additive effect when associated with pharmacological therapy with Sodium Diclofenac. The association of these therapies (pharmacology and photobiomodulation therapy) may be of interest in improving the inflammatory process in tendinous lesions.