Análise de biomarcadores circulantes modulados pela terapia de fotobiomodulação em indivíduos com trombose venosa profunda

Abstract

Venous thromboembolism (VTE) is a disease with high morbidity and mortality that includes pulmonary embolism (PE) and deep vein thrombosis (DVT). Deep vein thrombosis is a common disease and has little specific characteristics, which often makes its diagnosis difficult. Photobiomodulation therapy (PBM) has important anti-inflammatory effects such as reduction of chemical mediators such as cytokines, increased generation of nitric oxide (NO) and decreased migration of inflammatory cells. Changes in microRNA expression have recently been reported after PBM therapy. The aim of the project was to analyze the expression transcriptional elements such as messenger RNA (mRNA) and circulating microRNAs in subjects with DVT after irradiation with FBM therapy. Twenty subjects with subacute thrombosis in femoropopliteal territory and 10 healthy subjects as control were studied. DVT subjects were randomly separated into 2 groups of 10 individuals. Of these, only one group received PBM therapy. PBM therapy was applied at wavelength 660 nm. The peripheral blood of all subjects was collected before and after ultrasound and irradiation. The biomarker analyzes were performed with Real-Time PCR, in addition to Western blot analysis and measurement of the total NO concentration. We observed that individuals with DVT have lower expression of eNOS mRNA, higher expression of IL-6 and reduced concentration of NO compared to healthy individuals. However, after PBM therapy, subjects with DVT increased eNOS expression, decreased IL-6 expression, increased total NO concentration and modulated expression of circulating biomarkers such as miR-195 and mir-338. This is the first time that the effect of PBM therapy has been observed in subjects with subacute deep venous thrombosis, modulating transcriptional elements that can contribute to the management of the disease.