Análise do efeito do laser de baixa intensidade e da magnetoterapia em células musculares

Abstract

This study aims to understand and compare the effects of the low level laser therapy (LLLT) and magnetotherapy (MAG) on C2C12 cells of new passage (representing cells with 6 passages) and old (representing cells with 76 passages). For this study, C2C12 cells were cultured, with cell growth monitoring performed every 24 h. LLLT was used at a wavelength of 660 nm. In this study, it was employ new equipment, a magnet device, which induces an oscillating magnetic field, with 12 Hz rate. The effects of LLLT and MAG on cell viability and integrity were analyzed, in addition to the migration of cells by mechanical injury. In addition, the release of pro and anti-inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-10) was analyzed after the incubation of cells with LPS, by enzyme immunoassay. The expression of MyF-5, Pax7, MyoD, myogenin, caspase-3, p38 MAPK and NF-B proteins was analyzed using the western blot method. The intracellular calcium concentration and the concentration of the membrane potential in the cells were measured. The results showed an increase in cell viability and detachment in the two treatment methods used. In addition, it was observed an increase in cell migration after the using LLLT and MAG, when compared to natural migration. The expression of proteins in all myogenic factors evaluated denotes a significant increase by both LLLT and MAG. The group treated with LLLT was effective in both cell passages, in relation to the other groups, when the intracellular calcium concentration was assessed. The concentration of the membrane potential had a significant response post photobiomodulation and MAG. The LLLT, as well as MAG, reduced the release of the cytokines TNF-α, Il-1β and IL-6 in C2C12 cells, in the studied time and in the two cell passages evaluated. There was also a significant increase in the release of the cytokine IL-10. These data suggested that both treatments improved the process of migration and regeneration, facilitating the myogenic response, using the therapy with energy-based devices, as LLLT and MAG.