Análise do transcriptoma de RNA circulante e correlação com genes regulados no tecido cardíaco e cerebral em indivíduos com hipotireoidismo tratados com levotiroxina sódica

Abstract

Primary hypothyroidism is characterized by dysfunction of the thyroid gland, being one of the most common diseases and affecting approximately 10% of the world population. Thyroid dysfunction responds to repercussions in different tissues and the drug of choice used for replacement therapy or supplementation is levothyroxine sodium. Thyroid hormones can regulate gene transcription and impact an individual's metabolism and energy homeostasis. The objectives of this work were to identify circulating genes associated with the thyroid repercussions on cardiac functioning and on the brain, especially in mood. We performed the transcriptome of whole blood samples from eight individuals with hypothyroidism and four control subjects. The generated sequencing readings were aligned with the GRCh38 version. Subsequently, we made qualitative, quantitative analyzes in silico and using a cross-database with our libraries to detect patterns. We found genes expressed in cardiac and brain tissue, which had diseases that had repercussions in hypothyroidism. We observed that in thyroid dysfunction there may be regulation in several genes, in heart and brain, there is a tendency for signal oscillation when treated with levothyroxine, with greater stability in expression gain in untreated individuals.