Análise da expressão gênica por RNASeq transcriptoma para o desenvolvimento de biomarcadores entre gestantes saudáveis e com hipotireoidismo primário

Abstract

During pregnancy, under the endocrinological perspective, thyroid disorders are the most common in that period. However, they are ignored because of the non-specific nature of the symptoms and hypermetabolic state at this stage. Hypothyroidism in its clinical or subclinical, is similarly defined as outside of pregnancy. The thyroid stimulating hormone (TSH) acts as an important factor in the assessment of thyroid status in pregnancy. This disease has a prevalence important in pregnancy ranging from 1% to 1.5% and 5% and 8%, respectively on their clinical and subclinical. TSH reference values for pregnant women are different from the non-pregnant population and vary quarterly. The intense physiological changes of pregnancy influence the interpretation of thyroid function. This study aims to evaluate the profile of gene expression in the blood of women with gestational hypothyroidism and compare with the healthy pregnant women, and through RNA-Seq transcriptome define biomarkers for diagnosis of this disease more accurately. Fifty patients were evaluated, and selected these four in the control group and four for the hypothyroid group to perform the analysis. After statistical analysis, 11531 transcripts were found, and found loss of expression in 981 and gain 886. After the grouping of genes, we can see that the panel built with differentially expressed genes, allows to differentiate the group control group with hypothyroidism, forming a panel of biomarkers for the ability to differentiate euthyroid primary and subclinical hypothyroidism during pregnancy and also correlate them with serum TSH levels during pregnancy. The next step of this study will be the validation of these genes in a larger number of primary euthyroid and hypothyroid pregnant women by PCR in Real Time.