Análise da expressão de RNA mensageiro de genes relacionados ao metabolismo e sobrevivência celular no miocárdio de camundongos machos e fêmeas adultos submetidos à exposição pré-natal ao álcool

Abstract

Exposure to alcohol during pregnancy (EPA) is a worldwide public health problem. It is believed that alcohol favors the formation of cell death-induced teratogenicity due to an imbalance between the production of reactive oxygen species and endogenous antioxidants. Few studies have evaluated the long-term impact and cellular mechanisms involved in carriers of Fetal Alcohol Spectrum Disorders (FASD). Thus, further studies are needed to assess how molecular changes are caused by EPA. The aim of this study was to analyze the expression of messenger RNA (mRNA) of genes participating in the pathways of metabolism and cell survival in the myocardium. Thus, using 10 inbred C57Bl / 6 mice exposed to 10% alcohol (v / v), they were analyzed as mRNA expressions of component genes of access pathways and cellular metabolism (AKT-I, VEGFA, GAPDH, HK1, NDUFA3, PFKM, SLC2A1, TAZ and UCP2) in the myocardium of male animals and auction with prenatal exposure to alcohol. An EPA module for the expression of all responsible genes, including GAPDH which is recognized as the endogenous control gene in molecular reactions. Between males and betrothed, there was no difference in expression between 2 genes (HK1 and TAZ), however all other differences in gene expression related to angiogenesis, metabolism and cell survival between males and betrothed with EPA. In conclusion, our data found that EPA resulted in transcriptional changes that persisted in the myocardium into adulthood, being more pronounced in males than in the second. We also observed that, separately from EPA, there seems to be greater activation of cardioprotection in relation to the oxidative stress generated by alcohol.