Efeito do laser de baixa potência sobre células musculares C2C12 submetidas à lesão por veneno da serpente Bothrops jararacussu: mecanismos de reparo celular

Abstract

The effect of snake venom by Bothrops is associated with serius pathological manifestations and complex sites. Specific treatment for Bothrops accident is the u antivenom administration, which performs the neutralizing function by the greatest possible amount of venom circulating. Thus minimizing its systemic effects, but its response does not extend to the local manifestations. The aim of this study was to analyze the photobiomodulation effect (PMB) in C2C12 muscle cells subjected to injury by Bothrops Jararacussu venom therapy its cellular repair mechanism. For this, the cells were incubated with venom (12,5µg/mL) and immediately irradiated by PBM Aluminum Gallium Indium phosphorus and Aluminium gallium arsenide, wavelength (λ) 685 nm and 830 nm and the cells were incubated for 15, 30 and 60 minutes. After incubation periods, were analysed cell proliferation, intra and extracellular ATP levels, expression of myogenic regulatory factors, such as MyoD and myogenin, as well as cellular differentiation were analysed. Results demonstrated that unirradiated cells, the venom caused a decrease in cell proliferation, indicating myonecrosis. Infrared and red laser considerably decrease venom-induced cytotoxicity. Laser irradiation induced myoblasts to differentiate into myotubes and this effect were accompanied by an up MyoD, regulation specially myogenin. Moreover, LLLT was able to reduce the extracellular ATP while increased the intracellular ATP content past venom exposure. PMB did not modify the release of IL-6 induced by the venom. PBM caused a protective effect on C2C12 cells against the cytotoxicity caused by B. jararacussu venom, this as well and promoted these differentiation cells by up regulate myogenic factors.