Efeito da fotobiomodulação em modelo experimental in vitro da síndrome de sobreposição de asma e DPOC

Abstract

Asthma and Chronic Obstructive Pulmonary Disease (COPD) are chronic inflammatory pathologies of the airways with high prevalence nowadays. Patients who present features of both diseases can be triggered with the Asthma-COPD Overlap Syndrome (ACOS - Asthma-COPD Overlap Syndrome). Although this syndrome has been increasingly recognized, its clinical characteristics, diagnostic criteria and treatment are still not well defined. The objective of the study was to evaluate the effect of laser photobiomodulation (FMB) on the inflammatory process in an experimental in vitro model of ACOS. The study consisted of 4 groups: human bronchial epithelial cells (BEAS-2B) - negative control, BEAS-2B cells stimulated with cigarette smoke extract (CSE) + House Dust Mite (HDM) - positive control, BEAS-2B cells stimulated with EFC + HDM and treated with dexamethasone, BEAS-2B cells incubated with EFC + HDM and irradiated with laser (660nm, 30mW, 5.4J). After 24 hours of stimulation, cytokines in the cell culture supernatant were quantified. There was a reduction in the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, IL-4, IL-5, IL-13, IL-17, IL-21, IL-23, and an increase in IL- 10 and IFN-𝜸 in cells from the laser-irradiated ACOS group when compared to the positive control group. With these results, we can suggest that photobiomodulation acts in the modulation of inflammation observed in ACOS, and may be an option in the treatment of the disease.