Fotobiomodulação e seus efeitos na expressão de RNA mensageiro associado à hipóxia e inflamação em células endoteliais da veia umbilical humana

Abstract

Endothelial cells are cells that line the inside of blood and lymphatic vessels forming a layer called the endothelium, which, although for a long time it was considered as an inert layer, today is recognized as an important regulator of the main physiological functions. Under hypoxia, endothelial cells respond to chemical stimuli and secrete factors that modulate adaptive responses to cope with low oxygen availability and promote survival and vascular homeostasis. Our objective was to evaluate the effects of pretreatment with photobiomodulation on the transduction of hypoxic and inflammatory signals in human umbilical vein endothelial cells (HUVEC). For this, we used HUVEC cells in three experimental groups, control, cells with hypoxia and cells with hypoxia and treatment with photobiomodulation and evaluated the mRNA for the hypoxia and inflammation pathways. Our study highlighted that, based on the analysis of mRNA expression of genes involved in inflammation and adaptation to hypoxia, preconditioning with FBM was able to attenuate the inflammatory response induced by hypoxia, as evidenced by the significant reduction of pro-inflammatory cytokines (IL1 beta, IL6, and TNF alpha) and the increase in IL10 levels, an anti-inflammatory cytokine. Moreover, FBM treatment was also able to modulate the expression of hypoxia-regulating genes, such as HIF1 and EGLN1, suggesting that preconditioning can influence the cellular response to low oxygen concentrations.