Redução da expressão gênica de calicreínas tecidual e plasmática pela laserterapia em modelo de inflamação aguda induzida pela carragenina em ratos

Abstract

Background: Low level laser therapy (LLLT) has been used clinically, in order to treat inflammatory processes, where tissue and plasma prekallikrein have pivotal importance. Plasma prekallikrein (PPK) is synthesized in hepatocytes and secreted into the blood, where it participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. Tissue prekallikrein is associated with important diseased states (including cancer, inflammation, and neurodegeneration) and has been utilized or proposed as clinically important biomarker or therapeutic target of interest. Recently we demonstrated that both kinin receptors expression was altered after LLLT. Objective: To evaluate if LLLT modulates tissue and plasma prekallikreins mRNA expression in the carrageenan-induced rat paw edema. Methods: Experimental groups were designed as followed: A1 (Control-saline), A2 (Carrageenan-only), A3 (laser 660nm only) and A4 (Carrageenan + laser 660nm). Edema was measured by a plethysmometer. Subplantar tissue was collected for the quantification of prekallikreins mRNA by Real time-PCR. Results: A significantly decrease in the edema was observed after laser irradiation. Expression of prekallikreins is increased after carrageenan injection; however tissue and plasma prekallikrein mRNA expression significantly decreased after LLLT s 660nm wavelength. Conclusion: Our results suggest that expression of tissue and plasma prekallikreins is modulated by LLLT, possibly contributing to its anti-inflammatory effect.