Efeito do LED sobre a viabilidade de mioblastos cultivados na presença ou não de extrato de Melaleuca armillaris

Abstract

The skeletal muscle cells are constant target for damage the exercise, direct trauma, inflammatory processes, among others. In order to contain or reset the loss of muscle mass, begins a highly organized repair process with activation, proliferation and differentiation of satellite cells. The use of phototherapy resources that can promote faster and efficient repair as the Low Level Laser Therapy (LLLT) has shown good results in the regeneration of skeletal muscle. The use of herbal medicines has also been described in the literature and has shown promising results with regard to tissue repair. The objective of the study was to evaluate the effects of treatment with LED on cell viability, Nitric Oxide (NO) synthesis and total protein dosage in C2C12 muscle cells cultured with Melaleuca armillaris extract in differentiation medium. We analyzed eight experimental groups: (1) Control C2C12; (2) LED 0,2J; (3) LED 2,0J; (4) LED 4,0J; (5) 1% Melaleuca; (6) Melaleuca 1% + LED 0,2J; (7) Melaleuca 1% + LED 2,0J; (8) Melaleuca 1% + LED 4,0J. Cells received Melaleuca extract at a concentration of 1.0% (v/v) and were irradiated with infrared LED (850nm, 30mW power for 7, 67 and 134 seconds at full power 0,2J, and 2,0J 4,0J, respectively) according to the group. Cell viability was assessed by MTT method, the synthesis of NO by the Griess method and total protein determination was performed using the Bradford method after periods of 1, 2 and 3 days of incubation. The results showed that cell viability increased TLED the three periods energies and tested without changing the total concentration of protein and NO. Since the herbal remedy promoted a significant reduction in cell viability compared to control small a reduction in the dosage of NO after 24 hours and no change in the protein assay. However, the combined use of the two resources, the energy value of 0.2 and showed viability 2,0J, NO and protein similar to the group treated only with 1% Melaleuca a tendency to increase in these levels in the group irradiated with 2,0J energy. However, the group associated 4,0J showed a growth recovery in cell viability in the three periods analyzed and behavior similar to nitric oxide and total protein compared to the control. In conclusion, the TLED positively modulated the proliferation of C2C12 cells without deterioration in nitric oxide synthesis and total protein, while incubation with associated or not to TLED Melaleuca needs more specific analyzes to elucidate their contribution in this context.